山东五大地方鸡禽白血病病毒主要流行亚型鉴定及其分离株gp85基因的分子特征分析

doi:10.11843/j.issn.0366-6964.2014.03.015

Abstract

Abstract:

In order to identify different subtypies of avian leukosis virus (ALV) and analyze the sequence of gp85 gene，five ALV strains isolated from local chicken breeds in Shandong Province (Shouguang Chicken，Luhua Chicken，Bairi Chicken，Langya chicken，Luxi cock fighting) were amplified and sequenced.The prevailing subgroup of ALV and molecular characteristic of gp85 gene were identified.The sterile anticoagulant blood of Shouguang Chicken，Luhua Chicken，Bairi Chicken and the egg albumen from Langya chicken，Luxi cock fighting were inoculated onto CEF to culture nine to ten days.Proviral DNA was processed routinely.The gp85 and env genes were amplified by using primers subgroup J of ALV (ALV-J) gp85 (924 bp) and A-J group primers (encoding envelope protein containing env gene，2 400 bp).The specific products of PCR amplification were cloned，sequenced and analyzed.The study found that the five groups of local chickens from Shandong Province were infected with ALV-J during 2009 and 2011.The homology of the five ALV-J isolates was between 98.4% and 99.9%.Comparing with prototype strains HPRS-103，the homology was ranged from 97.8% to 98.3%.All of the five endogenous ALV (ALV-E) fragments were amplified，which has a great agreement with ev-1，ev-3，and ev-6 strains that cannot produce infectious virion.It is presumed that these fragments come from the chicken genome.Meanwhile，we also found that Langya and Luhua chicken were infected with subgroup A of ALV (ALV-A).This study showed that ALV-J existed in five groups of local chicken breeds in Shandong Province from 2009 to 2011.The co-infection of ALV-A and ALV-J was discovered in Langya chicken and Luhua chicken，while ALV-E fragments existed in all lines of five local chickens，which reveals that the purification work of ALV for local chicken breeds in Shandong Province will face more challenge.

CLC Number:

S852.659.3

CHEN Jing，WANG Bo，WANG Hai-ming，CHENG He-gang，WU Xing-chen，SUN Shu-hong. Identification of Main Epidemic Subtypies of Avian Leukosis Virus in Five Local Breeds in Shandong Province and Molecular Characteristics of Their gp85 Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(3): 451-457.

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Identification of Main Epidemic Subtypies of Avian Leukosis Virus in Five Local Breeds in Shandong Province and Molecular Characteristics of Their gp85 Gene

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